FastQCFastQC Report
星期二 11 七月 2023
GDM3_filtered.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGDM3_filtered.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8842403
Total Bases191.2 Mbp
Sequences flagged as poor quality0
Sequence length18-47
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT284844332.213449217367724No Hit
TCCTGTACTGAGCTGCCCCGAG90861210.275623040478928No Hit
TGAGGTAGTAGTTTGTGCTGTT1865942.1102182291397487No Hit
AAACCGTTACCATTACTGAGC1770502.0022837683376338No Hit
TATTGCACTTGTCCCGGCCTGTA1586921.7946705211241785No Hit
TCCTGTACTGAGCTGCCCCGAGA1567781.77302482142015No Hit
TCCTGTACTGAGCTGCCCCG1429161.6162574811394597No Hit
TCCTGTACTGAGCTGCCCCGA1340831.5163638210110983No Hit
TCAGTGCACTACAGAACTTTGT1152321.3031751662981206No Hit
AAACCGTTACCATTACTG1090361.233103716263554No Hit
TGAGGTAGTAGATTGTATAGTT1056731.1950710683509902No Hit
AAGGGCTTCCTCTCTGCAGGA1003091.1344088252933056No Hit
TATTGCACTTGTCCCGGCCTGT997941.1285846166477596No Hit
TCCTGTACTGAGCTGCCCCGAGT985711.1147535347574635No Hit
TGAGGTAGTAGTTTGTACAGTT976291.1041003220504653No Hit
TGAGGTAGTAGATTGTATAGT781830.8841827272518569No Hit
CGCGACCTCAGATCAGACGT616090.6967449911522919No Hit
TGAGGTAGTAGTTTGTACAGT471440.5331582376419622No Hit
TGAGGTAGTAGGTTGTGTGGTT467470.5286685078705415No Hit
CATTGCACTTGTCTCGGTCTGA465130.5260221684082935No Hit
AAACCGTTACCATTACTGAGA433790.4905793142429722No Hit
TCCTGTACTGAGCTGCCCCGAA425820.4815659272711275No Hit
CATTGCACTTGTCTCGGTCTG387110.4377882347140251No Hit
AAACCGTTACTATTACTGAGT378880.42848081002415295No Hit
TGAGGTAGTAGTTTGTGCTGT361870.4092439577793503No Hit
ATCCCACCGCTGCCACCA346530.3918957324157245No Hit
AAACCGTTACCATTACTGAG341250.385924504911165No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT304000.3437979472322173No Hit
TGAGGTAGTAGGTTGTATAGTT298810.3379285020146673No Hit
TTCAAGTAATCCAGGATAGGCT298720.3378267197276577No Hit
TGAGGGGCAGAGAGCGAGACT288480.3262461572945725No Hit
TGAGGTAGTAGGTTGTATAGT268640.30380881758046996No Hit
TATTGCACTTGTCCCGGCCTGTT248160.28064769271429946No Hit
CGCGACCTCAGATCAGACGTG242780.27456337377972934No Hit
AAACCGTTACCATTACTGAGTT232810.2632881582076727No Hit
CTATACGACCTGCTGCCTTT219850.24863150887829924No Hit
CTAGACTGAAGCTCCTTGAGG214540.24262635394473653No Hit
AATTGCACGGTATCCATCTGTA186470.2108815895407617No Hit
AGACCGTTACCATTACTGAGT165960.18768653724558812No Hit
CCCCCCACTGCTAAATTTGACTGGC164680.18623896694145245No Hit
AAACCGTTACCATTACTGGGT151930.17181980961510124No Hit
TGAGGTAGTAGGTTGTGTGGT148970.16847230328678756No Hit
ACATGGGTCAGTCGGTCCTGAG143950.16279511350025552No Hit
GAACCGTTACCATTACTGAGT142630.16130230662411563No Hit
TCCCCGGCACCTCCACCA142600.16126837919511247No Hit
CCGCACTGTGGGTACTTGCT141600.16013746489500646No Hit
AAACCGCTACCATTACTGAGT138710.15686912256770022No Hit
TGAGGTAGTAGTTTGTGCTGTC137420.15541024312056348No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTA129520.14647602014972627No Hit
AAACCGTTACCACTACTGAGT127570.1442707372645196No Hit
AGCAGCATTGTACAGGGCTATGA125790.14225770981033098No Hit
TCTCTCGGCTCCTCGCGGCTCG125120.14149999722925996No Hit
AAACCGTTACCATTACCGAGT121770.13771143432390495No Hit
AAGCCGTTACCATTACTGAGT119990.13569840686971632No Hit
TAACTATGACTCTCTTAAGG112830.12760106048095748No Hit
AAACCGTTACCATTACTGAGG109570.123914279862612No Hit
CGCGACCTCAGATCAGACGTGGC103700.11727581292098992No Hit
TTCAAGTAATTCAGGATAGGTT103670.11724188549198675No Hit
CTTCTCACTACTGCACTTGACTAGT103380.116913920344956No Hit
TTCAAGTAATCCAGGATAGG102730.11617882604988711No Hit
AAACCGTCACCATTACTGAGT101160.11440329059872073No Hit
TATTGCACTTGTCCCGGCCTG99080.1120509888545003No Hit
TCCTGTACTGAGCTGCCCCA99030.111994443139495No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC98210.11106709341340809No Hit
AAACCGTTGCCATTACTGAGT97000.10969868711027987No Hit
TGAGGTAGTAGTTTGTGCTG93740.10601190649193437No Hit
CGCGACCTCAGATCAGACGTGG91850.10387447846473406No Hit
AAACCGTTACCGTTACTGAGT90310.10213287044257087No Hit
AAACCGTTACCATCACTGAGT89440.10114897500147868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)