FastQCFastQC Report
星期二 11 七月 2023
Z1_filtered.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZ1_filtered.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10354433
Total Bases238.2 Mbp
Sequences flagged as poor quality0
Sequence length18-47
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT196960119.021814135066595No Hit
TCCTGTACTGAGCTGCCCCGAG141074013.624502664704094No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT4148074.006081260074791No Hit
TCCTGTACTGAGCTGCCCCGAGA3367903.252616536318309No Hit
TATTGCACTTGTCCCGGCCTGTA3357663.2427270522683376No Hit
TGAGGTAGTAGTTTGTGCTGTT3050112.9457045112948244No Hit
TCCTGTACTGAGCTGCCCCGAGT2421732.3388340047204905No Hit
TCCTGTACTGAGCTGCCCCGA2127632.054801069261832No Hit
TCCTGTACTGAGCTGCCCCG1728791.6696133916748508No Hit
AAGGGCTTCCTCTCTGCAGGA1431111.3821229998784097No Hit
TCAGTGCACTACAGAACTTTGT1318681.2735414870133401No Hit
TATTGCACTTGTCCCGGCCTGT1230431.1883122909772075No Hit
AAACCGTTACCATTACTGAGC1210951.1694990928040192No Hit
TGAGGTAGTAGTTTGTACAGTT1119861.0815271101759025No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATT904440.8734809525543311No Hit
TGAGGTAGTAGATTGTATAGTT805610.7780339106931302No Hit
CATTGCACTTGTCTCGGTCTGA774630.7481143583622589No Hit
TGAGGTAGTAGATTGTATAGT741500.7161184006888643No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC682780.6594083905898083No Hit
TCCTGTACTGAGCTGCCCCGAA663290.6405855347173525No Hit
TGAGGTAGTAGTTTGTACAGT605890.585150340921613No Hit
AAACCGTTACTATTACTGAGT552590.5336748038255692No Hit
TGAGGTAGTAGGTTGTGTGGTT551080.5322164912361691No Hit
AAACCGTTACCATTACTG509820.492368824058256No Hit
CATTGCACTTGTCTCGGTCTG508320.4909201691681234No Hit
TGAGGGGCAGAGAGCGAGACT504360.4870957202581735No Hit
CCCCCCACTGCTAAATTTGACTGGC496270.4792826415507252No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTA492290.4754388772422401No Hit
TATTGCACTTGTCCCGGCCTGTT431060.41630478462702886No Hit
CGCGACCTCAGATCAGACGT429380.4146822911500803No Hit
TGAGGTAGTAGTTTGTGCTGT374770.36194159544998744No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC339050.3274442936662973No Hit
TTCAAGTAATCCAGGATAGGCT335580.32409307202045734No Hit
AATTGCACGGTATCCATCTGTA324140.3130446640583796No Hit
CTAGACTGAAGCTCCTTGAGG312620.30191899450216153No Hit
TGAGGTAGTAGGTTGTATAGT311720.301049801568082No Hit
CTTCTCACTACTGCACTTGACTAGT302600.292241979836076No Hit
TCTCTCGGCTCCTCGCGGCTCG276250.2667939422660806No Hit
TGAGGTAGTAGGTTGTATAGTT264060.25502120685893664No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATT261350.25240397035743045No Hit
CCCCCCACTGCTAAATTTGACTGG249510.24096925442465078No Hit
CTATACGACCTGCTGCCTTT240400.23217109039191233No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT238380.23022023513986717No Hit
TGAGGTAGTAGTTTGTGCTGTC232700.22473466195589853No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGC224690.2169988448425906No Hit
AGCAGCATTGTACAGGGCTATGA200080.193231246945149No Hit
AAACCGTTACCATTACTGAGA178730.17261205900892881No Hit
TGAGGTAGTAGGTTGTGTGGT177630.1715497120894983No Hit
TCCTGTACTGAGCTGCCCCGAGC177290.17122135031440158No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA161640.15610705096068514No Hit
CCGCACTGTGGGTACTTGCT161570.1560394470658123No Hit
AGACCGTTACCATTACTGAGT146750.14172673675130254No Hit
ATCCCACCGCTGCCACCA145740.14075130912527994No Hit
AAACCGTTACCATTACTGAG144650.13969861990511698No Hit
AAACCGTTACCATTACTGAGTT140770.13595143258930742No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT139530.13475387788013116No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTA135260.1306300402928871No Hit
TCCTGTACTGAGCTGCCCCA133980.12939385478664067No Hit
TATTGCACTTGTCCCGGCCTG132910.12836048096501276No Hit
AGCTCGGTCTGAGGCCCCTCAGT130780.12630339102102453No Hit
AAACCGTTACCACTACTGAGT118870.11480107119337195No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA116460.1124735656698923No Hit
TGAGGGGCAGAGAGCGAGACTTT115320.11137258795339156No Hit
TATTGCACTTGTCCCGGCCTGA114800.11087038759147894No Hit
AAACCGTTACCATTACTGGGT114590.11066757590686037No Hit
TGGAGAGAAAGGCAGTTCCTGA112460.10861048596287214No Hit
TACCACAGGGTAGAACCACGGA112410.10856219746653438No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA111030.10722943496761243No Hit
GCGGGTGATGCGAACTGGAGTCTGAGC105820.10219777364921866No Hit
GAACCGTTACCATTACTGAGT105280.10167625788877092No Hit
CTATACGACCTGCTGCCTTTC104730.10114508442905565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)