FastQCFastQC Report
星期二 11 七月 2023
Z3_filtered.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZ3_filtered.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8946713
Total Bases230.7 Mbp
Sequences flagged as poor quality0
Sequence length18-47
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT250982828.05307379369384No Hit
TCCTGTACTGAGCTGCCCCGAG5125755.729199092448813No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3621844.048235368676742No Hit
AAACCGTTACCATTACTGAGC1793982.0051833561666723No Hit
TGAGGTAGTAGTTTGTGCTGTT1467511.6402783905105707No Hit
TGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAA1402291.5673801093205961No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTA1254721.4024368502711555No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATT1245591.3922319850877076No Hit
TCCTGTACTGAGCTGCCCCGAGA1111591.2424563076964692No Hit
TATTGCACTTGTCCCGGCCTGTA964121.0776248215406037No Hit
TCCTGTACTGAGCTGCCCCGA896091.0015857220411564No Hit
TCCTGTACTGAGCTGCCCCGAGT721110.806005512862657No Hit
TGAGGTAGTAGTTTGTACAGTT669800.7486548411690416No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC614920.6873138771747791No Hit
TATTGCACTTGTCCCGGCCTGT605050.6762818925788723No Hit
TCCTGTACTGAGCTGCCCCG590210.6596947951722605No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGC579540.6477686274277491No Hit
TGAGGTAGTAGATTGTATAGTT576350.6442030721226891No Hit
TCAGTGCACTACAGAACTTTGT574950.6426382516126314No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATT551790.6167516494605337No Hit
AAGGGCTTCCTCTCTGCAGGA464090.5187268217947754No Hit
TGAGGTAGTAGATTGTATAGT430260.4809140518981664No Hit
CCCCCCACTGCTAAATTTGACTGGC410900.45927481970193973No Hit
AAACCGTTACTATTACTGAGT372380.41621990109663737No Hit
AAACCGTTACCATTACTG355470.39731910479301163No Hit
AAACCGTTACCATTACTGAGTT352990.394547137032338No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC348060.38903673337906336No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT333290.3725278769979544No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTA327710.3662909495364387No Hit
TCCTGTACTGAGCTGCCCCGAA322320.3602663905727165No Hit
AAACCGTTACCATTACTGAGA318660.3561755026678513No Hit
TGAGGTAGTAGGTTGTGTGGTT313310.35019565286155935No Hit
TGAGGTAGTAGTTTGTACAGT292850.32732691883600157No Hit
TGAGGTAGTAGTTTGTGCTGT288260.32219654302088374No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT271690.30367577455541495No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT261510.29229729398942383No Hit
AAACCGTTACCATTACTGAG248590.2778562361394626No Hit
CATTGCACTTGTCTCGGTCTGA232030.25934664496335136No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA225210.25172373362149875No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTA223470.24977888527328418No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT216300.24176476880391715No Hit
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAG216120.24156357759548114No Hit
TATTGCACTTGTCCCGGCCTGTT206960.23132518054396067No Hit
AACAGCAGTTGAACATGGGTCAGTCGGTCCTGAG188280.2104460040240477No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT185750.20761814981658627No Hit
CGCGACCTCAGATCAGACGT182380.20385140330309018No Hit
TTCAAGTAATCCAGGATAGGCT181650.20303546117998866No Hit
TGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAG172920.19327768757084307No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT172830.19317709196662508No Hit
CCCCCCACTGCTAAATTTGACTGG171810.19203700845215443No Hit
TGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAG171230.19138872566941625No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAA169650.18962271395092253No Hit
TGAGGTAGTAGGTTGTATAGTT169250.18917562237662033No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA165090.18452587000387738No Hit
CAGTTGAACATGGGTCAGTCGGTCCTGAG165010.18443645168901696No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACA163550.18280456744281393No Hit
TGAGGGGCAGAGAGCGAGACT159730.17853484290822785No Hit
CTTCTCACTACTGCACTTGACTAGT156270.1746675007905138No Hit
AAACCGTTACCATTACTGGGT152410.1703530670984975No Hit
TGAGGTAGTAGGTTGTATAGT151840.16971596160511687No Hit
CATTGCACTTGTCTCGGTCTG148150.16559154183217903No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA146520.16376964366689756No Hit
GTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAG145060.1621377594206945No Hit
AGACCGTTACCATTACTGAGT143620.16052822975320655No Hit
AATTGCACGGTATCCATCTGTA136610.15269294991356044No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGC133630.149362117685009No Hit
GAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAG132460.14805437483017506No Hit
TGAGGTAGTAGTTTGTGCTGTC130110.1454277118311496No Hit
AAACCGCTACCATTACTGAGT125550.14033086788410448No Hit
ACAGCAGTTGAACATGGGTCAGTCGGTCCTGAG125450.14021909499052893No Hit
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATC121770.13610585250694865No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGT119060.1330768070910512No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA117380.13119902247898194No Hit
GAACCGTTACCATTACTGAGT116710.13045014409202577No Hit
AAACCGTTACCACTACTGAGT114770.12828174995666006No Hit
AAACCGTTACCATTACTGAGTTTAGT106760.11932874118125841No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT106380.11890400418567132No Hit
GTTGAACATGGGTCAGTCGGTCCTGAG104750.11708210602038983No Hit
AAACCGTTACCATTACCGAGT103800.11602026353142211No Hit
TGCTTGGACTACATATGGTTGAGGGTTGT102140.11416483349806796No Hit
CCCCCCACTGCTAAATTTGACTGGT101820.11380716023862618No Hit
CTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAA101350.1132818276388211No Hit
AAACCGTTACCATTACTGAGG100900.1127788496177311No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA97990.10952625841468258No Hit
TGAGGTAGTAGGTTGTGTGGT97150.10858736610864794No Hit
TACCACAGGGTAGAACCACGGA95730.10700019101987511No Hit
TGGAAGACTAGTGATTTTGTTGTT91330.10208218370255087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)